TMEM97 Polyklonaler Antikörper

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• KD/KO Validated

TMEM97 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 26444-1-AP

Synonyme

MAC30, Protein MAC30, S2R, Sigma2 receptor, Sigma-2 receptor

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  WB analysis using 26444-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26444-1-AP (TMEM97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26444-1-AP (TMEM97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 26444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26444-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293T-Zellen, HeLa-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

26444-1-AP bindet in WB, IHC, ELISA TMEM97 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM97 fusion protein Ag23917
• Vollständiger Name: transmembrane protein 97
• Beobachtetes Molekulargewicht: 19-20 kDa
• GenBank-Zugangsnummer: BC045655
• Gene symbol: TMEM97
• Gene ID (NCBI): 27346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Chem Biol; A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.; Authors - Yu-Shiuan Cheng
mouse	WB	ACS Chem Neurosci; Sigma Receptor Ligands Are Potent Antiprion Compounds that Act Independently of Sigma Receptor Binding; Authors - Robert C C Mercer ; KD Validated
mouse,human		Cell Signal; TMEM97 ablation aggravates oxidant-induced retinal degeneration.; Authors - Hongtao Shen ; KO Validated
human	WB	J Bioenerg Biomembr; Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling.; Authors - Yu Zhang
mouse	WB	Life Sci Alliance; The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth; Authors - Marina Badenes
human	WB,IHC	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen