TMEM9B Polyklonaler Antikörper

TMEM9B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24331-1-AP

Synonyme

C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B

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  WB analysis of rat liver using 24331-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 24331-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 24331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24331-1-AP bindet in WB, IHC, ELISA TMEM9B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM9B fusion protein Ag19383
• Vollständiger Name: TMEM9 domain family, member B
• Berechnetes Molekulargewicht: 198 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa, 35 kDa
• GenBank-Zugangsnummer: BC040124
• Gene symbol: TMEM9B
• Gene ID (NCBI): 56674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM9B, also termed as Transmembrane protein 9B encodes a 198 amino-acidprotein that contains an N-terminal signal peptide, a single transmembrane region, three potential N-glycosylation sites, and three conserved cys-rich domains in the N-terminus. There is a dimer form of TMEM9B protein (PMID: 12359240). TMEM9B mRNA is expressed in a wide range of tissues and cells. TMEM9B is a lysosomal transmembrane protein that regulates the activity of inflammatory signaling pathways.

Publikationen

Species	Application	Title
mouse		Nat Commun; Endosomal chloride/proton exchangers need inhibitory TMEM9 β-subunits for regulation and prevention of disease-causing overactivity; Authors - Rosa Planells-Cases