TMF1 Polyklonaler Antikörper

TMF1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22212-1-AP

Synonyme

TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa

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  WB analysis of mouse brain using 22212-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22212-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22212-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 22212-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22212-1-AP

  IP result of anti-TMF1 (IP:22212-1-AP, 4ug; Detection:22212-1-AP 1:5000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of human colon cancer using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22212-1-AP (TMF1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Jurkat-Zellen, Maus-Kolongewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

22212-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA TMF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMF1 fusion protein Ag17537
• Vollständiger Name: TATA element modulatory factor 1
• Berechnetes Molekulargewicht: 1093 aa, 123 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC126123
• Gene symbol: TMF1
• Gene ID (NCBI): 7110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMF1, also named as TMF and ARA160, is a potential coactivator of the androgen receptor. It mediates STAT3 degradation. The MW of TMF1 is about 160 kDa.

Publikationen

Species	Application	Title
human	WB,IF,IP,CoIP	Neurobiol Dis; The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors; Authors - Pankaj S Ghate