TMF1 Monoklonaler Antikörper

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TMF1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G12F2

Kat-Nr. : 67505-1-PBS

Synonyme

Androgen receptor-associated protein of 160 kDa, ARA160, TATA element modulatory factor, TMF

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  WB analysis using 67505-1-Ig (same clone as 67505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67505-1-Ig (same clone as 67505-1-PBS)

  WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67505-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TMF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TMF1 fusion protein Ag17666
• Vollständiger Name: TATA element modulatory factor 1
• Berechnetes Molekulargewicht: 1093 aa, 123 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC126123
• Gene symbol: TMF1
• Gene ID (NCBI): 7110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.