TMF1-Specific Polyklonaler Antikörper

TMF1-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19728-1-PBS

Synonyme

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  WB analysis using 19728-1-AP (same clone as 19728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 19728-1-AP (same clone as 19728-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 19728-1-AP (same clone as 19728-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

  IP result of anti-TMF1-Specific (IP:19728-1-AP, 4ug; Detection:19728-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 19728-1-AP (same clone as 19728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 19728-1-AP (same clone as 19728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19728-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TMF1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TATA element modulatory factor 1
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: NM_007114
• Gene symbol: TMF1
• Gene ID (NCBI): 7110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.