TMIGD1 Polyklonaler Antikörper

TMIGD1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27174-1-AP

Synonyme

TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164

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  WB analysis of mouse kidney using 27174-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 27174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27174-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 27174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27174-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27174-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of rat small intestine using 27174-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 27174-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Daudi-Zellen, HUVEC-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Ratten-Dünndarmgewebe, Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27174-1-AP bindet in WB, IHC, IF-P, ELISA TMIGD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMIGD1 fusion protein Ag25691
• Vollständiger Name: transmembrane and immunoglobulin domain containing 1
• Berechnetes Molekulargewicht: 262 aa, 29 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 29 kDa
• GenBank-Zugangsnummer: BC137201
• Gene symbol: TMIGD1
• Gene ID (NCBI): 388364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).

Publikationen

Species	Application	Title
mouse,human	WB,IF	Sci Signal; Intestinal brush border formation requires a TMIGD1-based intermicrovillar adhesion complex; Authors - Christian Hartmann
human	WB,IHC	Int Immunopharmacol; Macrophage-derived exosomes promote intestinal mucosal barrier dysfunction in inflammatory bowel disease by regulating TMIGD1 via mircroRNA-223; Authors - Xin Chang