TMIGD1 Polyklonaler Antikörper

TMIGD1 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27174-1-PBS

Synonyme

TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164

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  WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

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  WB analysis using 27174-1-AP (same clone as 27174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 27174-1-AP (same clone as 27174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

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  IF Staining of rat small intestine using 27174-1-AP (same clone as 27174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 27174-1-AP (same clone as 27174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27174-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA TMIGD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMIGD1 fusion protein Ag25691
• Vollständiger Name: transmembrane and immunoglobulin domain containing 1
• Berechnetes Molekulargewicht: 262 aa, 29 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 29 kDa
• GenBank-Zugangsnummer: BC137201
• Gene symbol: TMIGD1
• Gene ID (NCBI): 388364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).