TMIGD2/CD28H Rekombinanter Antikörper

TMIGD2/CD28H Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241807C12

Kat-Nr. : 84727-5-RR

Synonyme

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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  WB analysis using 84727-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig thymus using 84727-5-RR

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84727-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84727-5-RR against Human TMIGD2/CD28H were performed. The affinity constant is 1.23 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MOLT-4-Zellen, Hausschwein-Thymusgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84727-5-RR bindet in WB, ELISA TMIGD2/CD28H und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: transmembrane and immunoglobulin domain containing 2
• Berechnetes Molekulargewicht: 282 aa, 31 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC015655
• Gene symbol: TMIGD2
• Gene ID (NCBI): 126259
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821).