TMLHE Polyklonaler Antikörper

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• KD/KO Validated

TMLHE Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16621-1-AP

Synonyme

TMLD, TML-alpha-ketoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, EC:1.14.11.8

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  WB analysis of human liver using 16621-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16621-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16621-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 16621-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16621-1-AP (TMLHE antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, Jurkat-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below

Produktinformation

16621-1-AP bindet in WB, IHC, ELISA TMLHE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMLHE fusion protein Ag9919
• Vollständiger Name: trimethyllysine hydroxylase, epsilon
• Berechnetes Molekulargewicht: 376 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC025269
• Gene symbol: TMLHE
• Gene ID (NCBI): 55217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Trimethyllysine dioxygenase (TMLHE) encodes the first enzyme in carnitine biosynthesis, N6-trimethyllysine dioxygenase (TMLD) and enzyme deficiency was suggested to be a risk factor for ASD.

Publikationen

Species	Application	Title
human	WB	Cancer Discov; Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.; Authors - Chengheng Liao ; KD Validated
mouse	WB	Cell Rep; Inborn Errors of Long-Chain Fatty Acid β-Oxidation Link Neural Stem Cell Self-Renewal to Autism.; Authors - Zhigang Xie ; KD Validated
human	WB	Cell Chem Biol; Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite.; Authors - Aileen Mendoza
human,rabbit	WB	bioRxiv; αKG-mediated carnitine synthesis promotes homologous recombination via histone acetylation; Authors - Apoorva Uboveja
Human	WB	Nat Commun; BBOX1 restrains TBK1-mTORC1 oncogenic signaling in clear cell renal cell carcinoma; Authors - Chengheng Liao