TMOD1 Polyklonaler Antikörper

TMOD1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 10145-1-AP

Synonyme

Tropomodulin-1, tropomodulin 1, TMOD, E-Tmod, D9S57E

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  WB analysis using 10145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10145-1-AP (TMOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 10145-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10145-1-AP (TMOD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 10145-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10145-1-AP (TMOD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 10145-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using TMOD1 antibody (10145-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 10145-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using TMOD1 antibody (10145-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, mouse heart, mouse skeletal muscle, rat brain
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10145-1-AP bindet in WB, IHC, IF-P, ELISA TMOD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMOD1 fusion protein Ag0192
• Vollständiger Name: tropomodulin 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC002660
• Gene symbol: TMOD1
• Gene ID (NCBI): 7111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actin filaments control cell morphology. The length of these filaments is regulated in muscle and nonmuscle cell types by tropomodulins 1-4 (Tmod1-4), a family of proteins that cap the pointed ends of actin filaments. The molecular weight of Tmod 1 is about 40 kDa.

Publikationen

Species	Application	Title
mouse	WB	Glia; S1P1 deletion in oligodendroglial lineage cells: Effect on differentiation and myelination.; Authors - Danuta E Dukala
human,mouse,rat	WB	Commun Biol; Recessive TMOD1 mutation causes childhood cardiomyopathy; Authors - Catalina Vasilescu
human	IHC	Am J Surg Pathol; Identification of a unique gene expression signature that differentiates hepatocellular adenoma from well-differentiated hepatocellular carcinoma.; Authors - Chen Zong-Ming E ZM
mouse	IF	J Biol Chem; The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice.; Authors - Tomoki Ushijima
mouse	IHC	PLoS One; Expression and subcellular localization of mammalian formin Fhod3 in the embryonic and adult heart.; Authors - Kan-o Meikun M
mouse	IF	PLoS One; Transgenic Expression of the Formin Protein Fhod3 Selectively in the Embryonic Heart: Role of Actin-Binding Activity of Fhod3 and Its Sarcomeric Localization during Myofibrillogenesis.; Authors - Noriko Fujimoto

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