TMOD3 Polyklonaler Antikörper

TMOD3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12521-1-AP

Synonyme

Tropomodulin 3, Tropomodulin-3, UTMOD, U-Tmod

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  WB analysis of Jurkat using 12521-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12521-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12521-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12521-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12521-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12521-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12521-1-AP

  IP result of anti-TMOD3 (IP:12521-1-AP, 4ug; Detection:12521-1-AP 1:700) with Jurkat cells lysate 4000 ug.

• 

  IHC staining of human kidney using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Herzgewebe, humanes Nierengewebe, humanes Lungengewebe, Maushirngewebe, Mausnierengewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

12521-1-AP bindet in WB, IHC, IP, ELISA TMOD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMOD3 fusion protein Ag3215
• Vollständiger Name: tropomodulin 3 (ubiquitous)
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC020542
• Gene symbol: TMOD3
• Gene ID (NCBI): 29766
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomodulin 3(TMOD3) belongs to the tropomodulin family, which controls cytoskeletal dynamics by regulating the end-capping of actin filaments. TMOD3 bridges the gap between structural integrity and signaling cascades. TMOD3 regulates cytoskeletal architecture and is implicated in cell adhesion, migration, and mechanotransduction.

Publikationen

Species	Application	Title
human	WB	Carcinogenesis; MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3.; Authors - Hang Li
human	IHC	Comb Chem High Throughput Screen; Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma; Authors - Bin Zhong
human	WB,IHC	Drug Resist Updat; TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4; Authors - Zhiwei He