TMPO/LAP2 Monoklonaler Antikörper

TMPO/LAP2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G4E1

Kat-Nr. : 67157-1-Ig

Synonyme

LAP2, TMPO, 3G4E1, Lamina-associated polypeptide 2, isoforms beta/gamma, Thymopentin

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  WB analysis using 67157-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67157-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67157-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67157-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (67157-1-Ig, Clone: 3G4E1 ) at dilution of 1:2200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Leberkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67157-1-Ig bindet in WB, IHC, IF/ICC, ELISA TMPO/LAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TMPO/LAP2 fusion protein Ag6415
• Vollständiger Name: thymopoietin
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa, 39 kDa
• GenBank-Zugangsnummer: BC053675
• Gene symbol: TMPO/LAP2
• Gene ID (NCBI): 7112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).