TMPRSS3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TMPRSS3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17953-1-AP

Synonyme

DFNB10, DFNB8, ECHOS1, Serine protease TADG 12, TADG12, TMPRSS3

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Galerie der Validierungsdaten

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  WB analysis of mouse skin using 17953-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 17953-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 17953-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 17953-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 17953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17953-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 17953-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

17953-1-AP bindet in WB, IHC, IF, ELISA TMPRSS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMPRSS3 fusion protein Ag12435
• Vollständiger Name: transmembrane protease, serine 3
• Berechnetes Molekulargewicht: 453 aa, 49 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC074846
• Gene symbol: TMPRSS3
• Gene ID (NCBI): 64699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Gene Med; TMPRSS3 regulates cell viability and apoptosis processes of HEI-OC1 cells via regulation on circ-Slc4a2, miR-182, and AKT cascade.; Authors - Zhi-Wen Zhang ; KD Validated
mouse	IF	Mol Ther Methods Clin Dev; Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea; Authors - Ksenia A Aaron ; KO Validated