TMPRSS6 Polyklonaler Antikörper

TMPRSS6 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12950-1-AP

Synonyme

IRIDA, Matriptase 2, TMPRSS6

---

• 

  WB analysis of HEK-293 using 12950-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12950-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12950-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12950-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

12950-1-AP bindet in WB, IF, ELISA TMPRSS6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMPRSS6 fusion protein Ag4076
• Vollständiger Name: transmembrane protease, serine 6
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC039082
• Gene symbol: TMPRSS6
• Gene ID (NCBI): 164656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The type II transmembrane serine protease TMPRSS6 (also known as matriptase-2) controls iron homeostasis through its negative regulation of expression of hepcidin, a key hormone involved in iron metabolism. Upstream of the hepcidin-regulated signaling pathway, TMPRSS6 cleaves its target substrate hemojuvelin (HJV) at the plasma membrane(PMID:21724843).It Belongs to the peptidase S1 family.Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA).It has 4 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Haematologica; New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia.; Authors - Jing Liu
human,mouse	WB,IF	Cell Death Dis; Transmembrane serine protease 6, a novel target for inhibition of neuronal tumor growth; Authors - Yong Zuo ; KD Validated