Thymosin beta 4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Thymosin beta 4 Polyklonal Antikörper für IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 19850-1-AP

Synonyme

FX, PTMB4, Seraspenide, T beta 4, TB4X, THYB4, Thymosin beta 4, thymosin beta 4, X linked, Thymosin beta-4, TMSB4, TMSB4X

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Galerie der Validierungsdaten

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  IP experiment of mouse skeletal muscle using 19850-1-AP

  IP Result of anti-TMSB4X (IP:19850-1-AP, 4ug; Detection:19850-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (TMSB4X antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (TMSB4X antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 19850-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Thymosin beta 4 antibody (19850-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 19850-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Thymosin beta 4 antibody (19850-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

19850-1-AP bindet in WB, IP, IHC, IF, ELISA Thymosin beta 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Thymosin beta 4 fusion protein Ag13914
• Vollständiger Name: thymosin beta 4, X-linked
• Berechnetes Molekulargewicht: 63 aa, 7 kDa
• Beobachtetes Molekulargewicht: 5 kDa
• GenBank-Zugangsnummer: BC001631
• Gene symbol: TMSB4X
• Gene ID (NCBI): 7114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429).

Publikationen

Species	Application	Title
human	IHC	Nat Commun; Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma; Authors - Weilin Pu
chicken	IF	Elife; Single-cell proteomics reveals changes in expression during hair-cell development.; Authors - Ying Zhu
human	WB	J Mol Cell Biol; Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.; Authors - Zhou Yizhuo Y ; KD Validated
mouse	WB	Haematologica; Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.; Authors - Inga Scheller ; KO Validated
human	IHC,IF	Cancer Gene Ther; Lysine-40 succinylation of TAGLN2 induces glioma angiogenesis and tumor growth through regulating TMSB4X; Authors - Xiaoyi Zhang
human	IHC	Sci Rep; Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.; Authors - Li-Hsing Chi