TMUB1 Polyklonaler Antikörper

TMUB1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16638-1-AP

Synonyme

C7orf21, DULP, HOPS, SB144, TMUB1, Ubiquitin like protein DULP, Ubiquitin like protein SB144, UNQ763/PRO1555

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Galerie der Validierungsdaten

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  WB analysis using 16638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16638-1-AP (TMUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 16638-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using TMUB1 antibody (16638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, L02-Zellen
• Erfolgreiche Detektion in IF: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16638-1-AP bindet in WB, IF, ELISA TMUB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMUB1 fusion protein Ag9988
• Vollständiger Name: transmembrane and ubiquitin-like domain containing 1
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 27 kDa, 24 kDa
• GenBank-Zugangsnummer: BC000936
• Gene symbol: TMUB1
• Gene ID (NCBI): 83590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1), also referred to as hepatocyte odd protein shuttling (HOPS), is a transmembrane ubiquitin-like protein that was originally identified in the regenerated liver. HOPS is a transmembrane protein released into the cell by an enzymatic cleavage. This allows HOPS to be shuttled from the nucleus to the cytoplasm and vice versa, depending on cell cycle and environmental stress conditions. HOPS cDNA expression gives rise to the translation of three different proteins with distinct molecular weights of 27, 24 and 21 kDa, respectively.The three isoforms of HOPS are differentially expressed depending on the tissue being examined. In the brain, the shortest isoform prevails over the other, whereas in the intestine the higher molecular weight isoform prevails among the other two. Indeed, HOPS shows a different concentration of the three isoforms, which can play different cell-dependent functions. The ratio between different isoforms may vary owing to physiopathological changes in the cell (PMID:32860479).16638-1-AP can detect two bands and some literature also detected two bands (PMID:30610893, 29777440, 29967478)