TMX1 Polyklonaler Antikörper

TMX1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 27489-1-AP

Synonyme

Thioredoxin-related transmembrane protein 1, Thioredoxin domain-containing protein 1, PSEC0085, Protein disulfide-isomerase TMX1, PDIA11

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  WB analysis using 27489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27489-1-AP (TMX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27489-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27489-1-AP (TMX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27489-1-AP (TMX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27489-1-AP (TMX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27489-1-AP bindet in WB, IHC, IF, ELISA TMX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMX1 fusion protein Ag26595
• Vollständiger Name: thioredoxin-related transmembrane protein 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC036460
• Gene symbol: TMX1
• Gene ID (NCBI): 81542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMX1, one of the few transmembrane members of the family, is the first vascular thiol isomerase with antithrombotic function. It forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins while ignoring the same cysteine-containing ectodomains if not anchored at the ER membrane. As such, TMX1 is the first example of a topology-specific client protein redox catalyst in living cells(PMID: 30655304). Covalent modification with AMS increases the molecular mass of originally oxidized species by ~0.5 kDa per thiol group, which allows enhanced separation from the reduced form in electrophoresis(PMID: 29123984). TMX1 has 2 bands produces by redox with the MW of 30-32 kDa.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress.; Authors - Xie Ge
human	WB	Biochem J; Opposing regulation of endoplasmic reticulum retention under stress by ERp44 and PDIA6; Authors - Olaya Yassin
human	WB,IHC,IF	Sci Rep; Thioredoxin related transmembrane protein 1acts as a prognostic indictor and promotes proliferation and TMZ resistance of lower-grade glioma; Authors - Hongyan Hui ; KD Validated
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková
mouse	WB	J Thromb Haemost; A novel role for thioredoxin-related transmembrane protein TMX4 in platelet activation and thrombus formation; Authors - Zhenzhen Zhao
human	WB	Mol Oncol; Malformin C preferentially kills glioblastoma stem-like cells via concerted induction of proteotoxic stress and autophagic flux blockade; Authors - Emma Phillips