TMX1 Polyklonaler Antikörper

TMX1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27489-1-PBS

Synonyme

Thioredoxin-related transmembrane protein 1, Thioredoxin domain-containing protein 1, PSEC0085, Protein disulfide-isomerase TMX1, PDIA11

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  WB analysis using 27489-1-AP (same clone as 27489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27489-1-AP (TMX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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  WB analysis using 27489-1-AP (same clone as 27489-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27489-1-AP (TMX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27489-1-AP (same clone as 27489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27489-1-AP (TMX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27489-1-AP (same clone as 27489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27489-1-AP (TMX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27489-1-PBS bindet in WB, IHC, Indirect ELISA TMX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMX1 fusion protein Ag26595
• Vollständiger Name: thioredoxin-related transmembrane protein 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC036460
• Gene symbol: TMX1
• Gene ID (NCBI): 81542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMX1, one of the few transmembrane members of the family, is the first vascular thiol isomerase with antithrombotic function. It forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins while ignoring the same cysteine-containing ectodomains if not anchored at the ER membrane. As such, TMX1 is the first example of a topology-specific client protein redox catalyst in living cells(PMID: 30655304). Covalent modification with AMS increases the molecular mass of originally oxidized species by ~0.5 kDa per thiol group, which allows enhanced separation from the reduced form in electrophoresis(PMID: 29123984). TMX1 has 2 bands produces by redox with the MW of 30-32 kDa.