TMX4 Polyklonaler Antikörper

TMX4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21348-1-AP

Synonyme

UNQ475/PRO938, TXNDC13, Thioredoxin-related transmembrane protein 4, Thioredoxin domain-containing protein 13, PDIA14

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  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21348-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 21348-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21348-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21348-1-AP

  Immunofluorescent analysis of HepG2 cells using 21348-1-AP (TMX4 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A375-Zellen, humanes Herzgewebe, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

21348-1-AP bindet in WB, IHC, IF/ICC, ELISA TMX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMX4 fusion protein Ag15696
• Vollständiger Name: thioredoxin-related transmembrane protein 4
• Berechnetes Molekulargewicht: 349 aa, 39 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC033787
• Gene symbol: TMX4
• Gene ID (NCBI): 56255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4.

Publikationen

Species	Application	Title
	WB,IF	Nat Commun; TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress; Authors - Marika K Kucińska
human	WB	J Proteome Res; Mapping of the N-linked glycoproteome of human spermatozoa.; Authors - Wang Gaigai G
mouse	WB	JCI Insight; Protein disulfide isomerase is essential for spermatogenesis in mice; Authors - Yaqiong Zhang