TNC/Tenascin-C Rekombinanter Antikörper

TNC/Tenascin-C Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240844A2

Kat-Nr. : 83767-1-PBS

Synonyme

Tenascin, TNC, 240844A2, Glioma-associated-extracellular matrix antigen, GMEM

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  WB analysis of U-87 MG using 83767-1-RR (same clone as 83767-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83767-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83767-1-RR (same clone as 83767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83767-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83767-1-RR (same clone as 83767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83767-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83767-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83767-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA TNC/Tenascin-C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNC/Tenascin-C fusion protein Ag27122
• Vollständiger Name: tenascin C
• Berechnetes Molekulargewicht: 2201 aa, 241 kDa
• Beobachtetes Molekulargewicht: 220-350 kDa, 190-240 kDa
• GenBank-Zugangsnummer: BC151843
• Gene symbol: Tenascin C
• Gene ID (NCBI): 3371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.