TNC/Tenascin-C Polyklonaler Antikörper

TNC/Tenascin-C Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27789-1-PBS

Synonyme

Tenascin, TNC, Glioma-associated-extracellular matrix antigen, GMEM, GP 150 225

---

• 

  WB analysis using 27789-1-AP (same clone as 27789-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27789-1-AP (same clone as 27789-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

27789-1-PBS bindet in WB, IHC, Indirect ELISA TNC/Tenascin-C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNC/Tenascin-C fusion protein Ag27122
• Vollständiger Name: tenascin C
• Berechnetes Molekulargewicht: 2201 aa, 241 kDa
• Beobachtetes Molekulargewicht: 220-350 kDa, 190-240 kDa
• GenBank-Zugangsnummer: BC151843
• Gene symbol: Tenascin C
• Gene ID (NCBI): 3371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.