TNC/Tenascin-C Rekombinanter Antikörper

TNC/Tenascin-C Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240844A2

Kat-Nr. : 83767-1-RR

Synonyme

Tenascin, TNC, Hexabrachion, GP 150-225, GP 150 225

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  WB analysis of U-87 MG using 83767-1-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83767-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83767-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83767-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83767-1-RR bindet in WB, IHC, ELISA TNC/Tenascin-C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNC/Tenascin-C fusion protein Ag27122
• Vollständiger Name: tenascin C
• Berechnetes Molekulargewicht: 2201 aa, 241 kDa
• Beobachtetes Molekulargewicht: 220-350 kDa, 190-240 kDa
• GenBank-Zugangsnummer: BC151843
• Gene symbol: Tenascin C
• Gene ID (NCBI): 3371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.