TNF-alpha Monoklonaler Antikörper

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• KD/KO Validated

TNF-alpha Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

7B8A11

Kat-Nr. : 60291-1-PBS

Synonyme

TNF, TNF alpha, TNF-a, 7B8A11, C-domain 1

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  WB analysis using 60291-1-Ig (same clone as 60291-1-PBS)

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody 60004-1-Ig as loading control. This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60291-1-Ig (same clone as 60291-1-PBS)

  WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells. This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 60291-1-Ig (same clone as 60291-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using TNF-alpha antibody (60291-1-Ig, Clone: 7B8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60291-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA TNF-alpha und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNF-alpha fusion protein Ag11413
• Vollständiger Name: tumor necrosis factor (TNF superfamily, member 2)
• Berechnetes Molekulargewicht: 233 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC028148
• Gene symbol: TNF-alpha
• Gene ID (NCBI): 7124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.