TNF Alpha Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TNF Alpha Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte und mehr (5)

Anwendung

WB, RIP, IHC, IF, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

364

CloneNo.

7B8A11

Rezensionen (2)

Kat-Nr. : 60291-1-Ig

Synonyme

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

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Galerie der Validierungsdaten

• 

  WB analysis using 60291-1-Ig

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody 60004-1-Ig as loading control.

• 

  WB analysis of HeLa using 60291-1-Ig

  WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells.

• 

  WB analysis using 60291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60291-1-Ig

  Untreted and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60291-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60291-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 60291-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 60291-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60291-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 60291-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 60291-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HeLa-Zellen, HL-60-Zellen, PC-12-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 261 publications below
• IHC: See 86 publications below
• IF: See 42 publications below
• ELISA: See 4 publications below
• RIP: See 1 publications below

Produktinformation

60291-1-Ig bindet in WB, RIP, IHC, IF, Cell treatment, ELISA TNF Alpha und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNF Alpha fusion protein Ag11413
• Vollständiger Name: tumor necrosis factor (TNF superfamily, member 2)
• Berechnetes Molekulargewicht: 233 aa, 26 kDa
• Beobachtetes Molekulargewicht: 17-22 kDa
• GenBank-Zugangsnummer: BC028148
• Gene symbol: TNF
• Gene ID (NCBI): 7124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Publikationen

Species	Application	Title
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
human	IHC	Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Blood; The hepatocyte-specific HNF4α/miR-122 pathway contributes to iron overload-mediated hepatic inflammation.; Authors - Min Li
human,rat	WB	ACS Nano; Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis; Authors - Xia Niu
mouse,rat	WB	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
mouse	WB	Brain Behav Immun; Oxytocin alleviates cognitive and memory impairments by decreasing hippocampal microglial activation and synaptic defects via OXTR/ERK/STAT3 pathway in a mouse model of sepsis-associated encephalopathy; Authors - Junliang Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (04-19-2023)

Worked well for WB in U2OS cells at 1:1000. TNF-alpha observed in upper membrane part

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

TNF Alpha Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:60291-1-Ig)

FH

Feng-Qian (Verified Customer) (12-07-2018)

Signal was very weak.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/250
• Cell Tissue Type: HaCaT

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