TNF-alpha Polyklonaler Antikörper

TNF-alpha Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Kat-Nr. : 26405-1-AP

Synonyme

TNF, TNF alpha, TNF-a, C-domain 1, C-domain 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen
Erfolgreiche Detektion in IHChumanes Ovarialkarzinomgewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCPMA, LPS and Brefeldin A treated THP-1 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

26405-1-AP bindet in WB, IHC, IF/ICC, ELISA TNF-alpha und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Kaninchen, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TNF-alpha fusion protein Ag24020
Vollständiger Name tumor necrosis factor (TNF superfamily, member 2)
Berechnetes Molekulargewicht 233 aa, 26 kDa
Beobachtetes Molekulargewicht26 kDa
GenBank-ZugangsnummerBC028148
Gene symbol TNF-alpha
Gene ID (NCBI) 7124
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TNF-alpha antibody 26405-1-APProtokoll herunterladen
IHC protocol for TNF-alpha antibody 26405-1-APProtokoll herunterladenl
IF protocol for TNF-alpha antibody 26405-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

J Control Release

Efficient gene delivery by multifunctional star poly (β-amino ester)s into difficult-to-transfect macrophages for M1 polarization

Authors - Tao Bo
mouseIHC

J Nanobiotechnology

Oxygen-delivery nanoparticles enhanced immunotherapy efficacy monitored by granzyme B PET imaging in malignant tumors

Authors - Xingyi Wang
mouseIHC

Redox Biol

Ethyl carbamate triggers ferroptosis in liver through inhibiting GSH synthesis and suppressing Nrf2 activation.

Authors - Yang Xu
ratIHC

Cell Prolif

Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Authors - Lihua Luo
mouse

Int Immunopharmacol

Emu oil alleviates atopic dermatitis-like responses by inhibiting Cdc42 signaling of keratinocyte

Authors - Lingwei Bu
ratIHC

Neurochem Res

BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway

Authors - Xiaojin Wei