TNF-alpha Rekombinanter Antikörper

TNF-alpha Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

5H8

Kat-Nr. : 82863-3-PBS

Synonyme

Tnf, Cachectin, TNF alpha, TNF alpha,TNF-a,TNF-alpha,TNFα, TNF α

---

• 

  Sandwich ELISA standard curve of MP02049-1

  Sandwich ELISA standard curve of MP02049-1, Rat TNF-alpha Recombinant Matched Antibody Pair - PBS only. 82863-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31374. 82863-7-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL.

• 

  Sample test of MP02049-1

  Rat spleens were cut into 1-2 mm pieces and cultured unstimulated or stimulated with 5 μg/mL ConA for 2 days. The mean TNF-alpha concentration was not determined in unstimulated supernatant, 118.8 pg/mL in ConA stimulated supernatant.

• 

  Affinity and Kinetic Characterization of 82863-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 82863-3-PBSagainst Rat TNF-alpha was performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

82863-3-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test TNF-alpha und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNF-alpha fusion protein Eg31374
• Vollständiger Name: tumor necrosis factor (TNF superfamily, member 2)
• GenBank-Zugangsnummer: BC107671
• Gene symbol: TNF-alpha
• Gene ID (NCBI): 24835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.