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TNF-alpha Rekombinanter Antikörper

TNF-alpha Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240668B4

Kat-Nr. : 83721-7-PBS

Synonyme

TNF, TNF alpha, TNF-a, 240668B4, C-domain 1

Filter:
  • Western Blot (WB) analysis of THP-1 cells using TNF-alpha Recombinant antibody (83721-7-RR)

    WB analysis of THP-1 using 83721-7-RR (same clone as 83721-7-PBS)

    Untreated, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83721-7-RR (TNF-alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83721-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83721-7-RR

    Affinity and Kinetic Characterization of 83721-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83721-7-RR against Human TNF-alpha were performed. The affinity constant is 8.51 nM.

Geprüfte Anwendungen

Produktinformation

83721-7-PBS bindet in WB, ELISA TNF-alpha und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name tumor necrosis factor (TNF superfamily, member 2)
Berechnetes Molekulargewicht26 kDa
Beobachtetes Molekulargewicht26 kDa
GenBank-ZugangsnummerNM_000594.4
Gene symbol TNF-alpha
Gene ID (NCBI) 7124
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.