TNF-alpha Rekombinanter Antikörper

TNF-alpha Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240668B11

Kat-Nr. : 98064-1-RR

Synonyme

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

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  FC experiment of human PBMCs using 98064-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

• 

  FC experiment of human PBMCs using 98064-1-RR

  1x10^6 untreated(black) or PMA, Ionomycin and Brefeldin A treated(red) human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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  Affinity and Kinetic Characterization of 98064-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98064-1-RR against Human TNF-alpha were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98064-1-RR bindet in FC (Intra) TNF-alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: tumor necrosis factor (TNF superfamily, member 2)
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM_000594.4
• Gene symbol: TNF-alpha
• Gene ID (NCBI): 7124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.