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TNF Alpha Polyklonaler Antikörper

TNF Alpha Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (4)

Anwendung

WB, Transwell Assay, Cell treatment, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17590-1-AP

Synonyme

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using TNF Alpha Polyclonal antibody (17590-1-AP)

    WB analysis using 17590-1-AP

    Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TNF Alpha Polyclonal antibody (17590-1-AP)

    WB analysis of RAW 264.7 using 17590-1-AP

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen, mit LPS behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 435 publications below
ELISASee 3 publications below

Produktinformation

17590-1-AP bindet in WB, Transwell Assay, Cell treatment, ELISA TNF Alpha und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Fisch, Kaninchen, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TNF Alpha fusion protein Ag11433
Vollständiger Name tumor necrosis factor (TNF superfamily, member 2)
Berechnetes Molekulargewicht 233 aa, 26 kDa
Beobachtetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC028148
Gene symbol TNF
Gene ID (NCBI) 7124
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protokolle

Produktspezifische Protokolle
WB protocol for TNF Alpha antibody 17590-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

HMGB1 mediates depressive behavior induced by chronic stress through activating the kynurenine pathway.

Authors - Bo Wang
View Article
mouseWB

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
View Article
WB

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
View Article
human

Mater Today Bio

Dexamethasone-loaded zeolitic imidazolate frameworks nanocomposite hydrogel with antibacterial and anti-inflammatory effects for periodontitis treatment

Authors - Ning Li
View Article
mouseWB

Acta Biomater

Mild hyperthermia promotes immune checkpoint blockade-based immunotherapy against metastatic pancreatic cancer using size-adjustable nanoparticles.

Authors - Qianwen Yu
View Article
mouseWB

Redox Biol

Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.

Authors - Guoqing Wang
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Margarita (Verified Customer) (11-22-2023)

Prostate Cancer cell line, overnight incubation, Mouse

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: TRAMPC C2
TNF Alpha Antibody Immunofluorescence validation (1:100 dilution) in TRAMPC C2 (Cat no:17590-1-AP)
FH

Isha (Verified Customer) (09-20-2021)

signal was very good. My sample was renal tissue lysate after ischemia reperfusion

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: Kidney