TNFAIP3 Polyklonaler Antikörper

TNFAIP3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 23456-1-AP

Synonyme

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37

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  WB analysis of Jurkat using 23456-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23456-1-AP (TNFAIP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23456-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23456-1-AP (TNFAIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23456-1-AP (TNFAIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

23456-1-AP bindet in WB, IHC, IF, ELISA TNFAIP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFAIP3 fusion protein Ag18150
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 3
• Berechnetes Molekulargewicht: 790 aa, 90 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa, 37-40 kDa
• GenBank-Zugangsnummer: BC114480
• Gene symbol: TNFAIP3
• Gene ID (NCBI): 7128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Upon T- or B-cell receptor engagement human (h) A20 is cleaved by MALT1 after arginine 439, yielding an N-terminal fragment and a C-terminal one (~37-40 kDa). This antibody, which is a rabbit polyclonal antibody raised against 350 aa of C-terminus of human TNFAIP3, can recognize full-length A20 (~90 kDa) and cleaved form (~37-40 kDa).

Publikationen

Species	Application	Title
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human,mouse	WB,IHC	Am J Cancer Res; ZY-444 inhibits the growth and metastasis of prostate cancer by targeting TNFAIP3 through TNF signaling pathway; Authors - Meng-Ting Han
mouse	WB	Biomedicines; The Cholinergic Anti-Inflammatory Pathway Attenuates the Development of Atherosclerosis in Apoe-/- Mice through Modulating Macrophage Functions.; Authors - Zhengjiang Qian
		Biomedicines; Sertoli Cells Express Accommodation, Survival, and Immunoregulatory Factors When Exposed to Normal Human Serum; Authors - Rachel L Washburn
rat	WB	Int Immunopharmacol; TNFAIP3-upregulated RIP3 exacerbates acute pancreatitis via activating NLRP3 inflammasome.; Authors - Yifan Wang
human	WB	Mol Cell Biol; LncRNA HOXA-AS3 integrates NF-κB signaling to regulate endothelium inflammation.; Authors - Xinxing Zhu