TSG-6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSG-6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13321-1-AP

Synonyme

Hyaluronate binding protein, TNF alpha induced protein 6, TNF stimulated gene 6 protein, TNFAIP6, TSG 6, TSG6, TSG-6

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 13321-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13321-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: Rattennierengewebe, humanes Ovarialkarzinomgewebe, humanes Zervixkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13321-1-AP bindet in WB, IHC, IF, ELISA TSG-6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSG-6 fusion protein Ag3885
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 6
• Berechnetes Molekulargewicht: 277 aa, 31 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC030205
• Gene symbol: TNFAIP6
• Gene ID (NCBI): 7130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	JACC Basic Transl Sci; Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6.; Authors - Rena Watanabe
mouse	WB	Front Pharmacol; TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation.; Authors - Yue-Meng Wan
rat	WB	Neurochem Res; Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage.; Authors - Bin Tang
human	WB,IHC	Contrast Media Mol Imaging; TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway; Authors - Hongquan Cui ; KD Validated
human	WB,IHC,IF	Neural Regen Res; Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis; Authors - Mingxiang Ding