TNFAIP8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TNFAIP8 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 15790-1-AP

Synonyme

GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8

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Galerie der Validierungsdaten

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  WB analysis using 15790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15790-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 15790-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, MOLT-4-Zellen, PC-13-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

15790-1-AP bindet in WB, IHC, IF, ELISA TNFAIP8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFAIP8 fusion protein Ag8491
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 8
• Berechnetes Molekulargewicht: 198 aa, 23 kDa
• Beobachtetes Molekulargewicht: 19-23 kDa
• GenBank-Zugangsnummer: BC007014
• Gene symbol: TNFAIP8
• Gene ID (NCBI): 25816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested. There are four major isoforms of TNFAIP8.

Publikationen

Species	Application	Title
	WB	J Immunol; Crucial Roles of TNFAIP8 Protein in Regulating Apoptosis and Listeria Infection.; Authors - Thomas P Porturas
human	WB	Oncotarget; TNFAIP8 promotes prostate cancer cell survival by inducing autophagy.; Authors - Suryakant Niture ; KD Validated
human	WB	Toxins (Basel); Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis; Authors - Suryakant Niture
human	IHC	J Proteome Res; Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.; Authors - Jing Xiao ; KD Validated
mouse	IF	Sci Rep; The TIPE Molecular Pilot That Directs Lymphocyte Migration in Health and Inflammation.; Authors - Honghong Sun ; KO Validated
mouse	WB	Sci Rep; The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.; Authors - Ji-Soo Kim ; KD Validated