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TNFR1/CD120a Polyklonaler Antikörper

TNFR1/CD120a Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(75)

Kat-Nr. : 21574-1-AP

Synonyme

TNFRSF1A, TBP1, p60, p55 R, p55



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe
Erfolgreiche Detektion in IHChumanes Hirngewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21574-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ELISA TNFR1/CD120a und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TNFR1/CD120a fusion protein Ag16112
Vollständiger Name tumor necrosis factor receptor superfamily, member 1A
Berechnetes Molekulargewicht 455 aa, 50 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC010140
Gene symbol TNFR1
Gene ID (NCBI) 7132
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TNFR1/CD120a antibody 21574-1-APProtokoll herunterladen
IHC protocol for TNFR1/CD120a antibody 21574-1-APProtokoll herunterladenl
IF protocol for TNFR1/CD120a antibody 21574-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang
WB,IF

Adv Healthc Mater

Hyaluronic Acid-based ROS-responsive Multifunctional Injectable Hydrogel Platform Accelerating Diabetic Wound Healing

Authors - Chen Shi
humanWB

Aging Dis

MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.

Authors - Yanhui Cui
mouseWB

PLoS Biol

A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.

Authors - Yingying Zhang
humanWB

Cell Death Discov

Repositioning linifanib as a potent anti-necroptosis agent for sepsis

Authors - Liang Yu
humanWB,IP

Cancer Lett

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Authors - Bei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xinda (Verified Customer) (08-16-2024)

It gave very nice and specific bands.

  • Applications: Cell culture
FH

Tinne (Verified Customer) (08-14-2023)

This AB worked quite well - not the strongest signal - might be worth increasing the concentration. I got the best results when I did not permeabilise the cells.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Human hippocampal progenitor cells
TNFR1/CD120a Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:21574-1-AP)