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TNFR1/CD120a Polyklonaler Antikörper
TNFR1/CD120a Polyklonal Antikörper für WB, IHC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (2)
Anwendung
WB, IHC, IF-P, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 21574-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Raji-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe |
Erfolgreiche Detektion in IHC | humanes Hirngewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Maushirngewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 62 publications below |
IHC | See 11 publications below |
IF | See 17 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Produktinformation
21574-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ELISA TNFR1/CD120a und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | TNFR1/CD120a fusion protein Ag16112 |
Vollständiger Name | tumor necrosis factor receptor superfamily, member 1A |
Berechnetes Molekulargewicht | 455 aa, 50 kDa |
Beobachtetes Molekulargewicht | 50 kDa |
GenBank-Zugangsnummer | BC010140 |
Gene symbol | TNFR1 |
Gene ID (NCBI) | 7132 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for TNFR1/CD120a antibody 21574-1-AP | Protokoll herunterladen |
IHC protocol for TNFR1/CD120a antibody 21574-1-AP | Protokoll herunterladenl |
IF protocol for TNFR1/CD120a antibody 21574-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies | ||
Adv Healthc Mater Hyaluronic Acid-based ROS-responsive Multifunctional Injectable Hydrogel Platform Accelerating Diabetic Wound Healing | ||
Aging Dis MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis. | ||
PLoS Biol A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5. | ||
Cancer Lett Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xinda (Verified Customer) (08-16-2024) | It gave very nice and specific bands.
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FH Tinne (Verified Customer) (08-14-2023) | This AB worked quite well - not the strongest signal - might be worth increasing the concentration. I got the best results when I did not permeabilise the cells.
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