TNFR1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TNFR1 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

CloneNo.

2A6E3

Kat-Nr. : 60192-1-Ig

Synonyme

CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A

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Galerie der Validierungsdaten

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  WB analysis using 60192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 60192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of Raji using 60192-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 60192-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 60192-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 60192-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1 antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Raji using 60192-1-Ig

  1X10^6 Raji cells were stained with 0.2ug TNFR1 antibody (60192-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, A549-Zellen, Daudi-Zellen, HeLa-Zellen, HL-60-Zellen, Kaninchenhirngewebe, Maushirngewebe, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

60192-1-Ig bindet in WB, IHC, IF, FC, ELISA TNFR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNFR1 fusion protein Ag16112
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1A
• Berechnetes Molekulargewicht: 455 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC010140
• Gene symbol: TNFRSF1A
• Gene ID (NCBI): 7132
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Publikationen

Species	Application	Title
human	WB	Mol Ther; Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities.; Authors - Yuechao Zhao
mouse	WB,IF	Front Immunol; Single Cell Dissection of Epithelial-Immune Cellular Interplay in Acute Kidney Injury Microenvironment.; Authors - Min Zhang ; KD Validated
human	WB	J Transl Med; CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.; Authors - Kun Lin
mouse	IF	Life Sci; Comparative transcriptome and Lipidome analyses suggest a lipid droplet-specific response to heat exposure of brown adipose tissue in normal and obese mice.; Authors - Qiankun Wang
		Inflammation; Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion.; Authors - Qian Chen
human	WB	Oncotarget; Inflammatory factor TNF-α promotes the growth of breast cancer via the positive feedback loop of TNFR1/NF-κB (and/or p38)/p-STAT3/HBXIP/TNFR1.; Authors - Xiaoli Cai

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