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TNFR1/CD120a Monoklonaler Antikörper

TNFR1/CD120a Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

CloneNo.

2A6E3

Kat-Nr. : 60192-1-Ig

Synonyme

CD120a, TNFR1, TNFRSF1A, 2A6E3, FPF



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHausschwein-Hirngewebe, A549-Zellen, Daudi-Zellen, HeLa-Zellen, HL-60-Zellen, Maushirngewebe, Kaninchenhirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Leberkarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60192-1-Ig bindet in WB, IHC, IF-P, ELISA TNFR1/CD120a und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TNFR1/CD120a fusion protein Ag16112
Vollständiger Name tumor necrosis factor receptor superfamily, member 1A
Berechnetes Molekulargewicht 455 aa, 50 kDa
Beobachtetes Molekulargewicht 50-55 kDa
GenBank-ZugangsnummerBC010140
Gene symbol TNFR1
Gene ID (NCBI) 7132
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TNFR1/CD120a antibody 60192-1-IgProtokoll herunterladen
IHC protocol for TNFR1/CD120a antibody 60192-1-IgProtokoll herunterladenl
IF protocol for TNFR1/CD120a antibody 60192-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Ther

Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities.

Authors - Yuechao Zhao
human

Int Immunopharmacol

EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis

Authors - Huiling Zhang
mouseWB,IF

Front Immunol

Single Cell Dissection of Epithelial-Immune Cellular Interplay in Acute Kidney Injury Microenvironment.

Authors - Min Zhang
  • KD Validated
humanWB

J Transl Med

CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.

Authors - Kun Lin
mouseIF

Life Sci

Comparative transcriptome and Lipidome analyses suggest a lipid droplet-specific response to heat exposure of brown adipose tissue in normal and obese mice.

Authors - Qiankun Wang

Inflammation

Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion.

Authors - Qian Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lianna (Verified Customer) (06-12-2025)

Rabbit TNFR1 antibody (1:1000) did not work well for IF or WB in U2OS cells. In IF it solely stained microtubules and for WB it was poor as well with no clear bands.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS