TNFR1/CD120a Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TNFR1/CD120a Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A6E3

Kat-Nr. : 60192-1-PBS

Synonyme

CD120a, TNFR1, TNFRSF1A, 2A6E3, FPF

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  WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1/CD120a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 60192-1-Ig (same clone as 60192-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 60192-1-Ig (same clone as 60192-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1/CD120a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1/CD120a antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60192-1-PBS bindet in WB, IHC, IF-P, ELISA TNFR1/CD120a und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNFR1/CD120a fusion protein Ag16112
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1A
• Berechnetes Molekulargewicht: 455 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC010140
• Gene symbol: TNFR1
• Gene ID (NCBI): 7132
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.