TNFR1/CD120a Rekombinanter Antikörper

TNFR1/CD120a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250007H7

Kat-Nr. : 98498-1-RR

Synonyme

CD120a, Tnfrsf1a, TNF alphaR1, TNF R, TNF R I

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  FC experiment of Transfected HEK-293T using 98498-1-RR

  1x10^6 HEK-293T cells or TNFR1/CD120a transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse TNFR1/CD120a Rabbit RecAb (98498-1-RR, Clone: 250007H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 98498-1-RR

  1x10^6 HEK-293T cells or TNFR1/CD120a transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse TNFR1/CD120a Rabbit RecAb (98498-1-RR, Clone: 250007H7) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98498-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98498-1-RR against Mouse TNFR1/CD120a were performed. The affinity constant is 17.7 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98498-1-RR bindet in FC TNFR1/CD120a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNFR1/CD120a fusion protein Eg0733
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1a
• Berechnetes Molekulargewicht: 50KDa
• GenBank-Zugangsnummer: NM_011609.4
• Gene symbol: Tnfrsf1a
• Gene ID (NCBI): 21937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.