TNFR2 / TNFRSF1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TNFR2 / TNFRSF1B Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (1)

Kat-Nr. : 19272-1-AP

Synonyme

CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

---

Galerie der Validierungsdaten

• 

  WB analysis using 19272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19272-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19272-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19272-1-AP

  IP Result of anti-TNFR2 / TNFRSF1B (IP:19272-1-AP, 4ug; Detection:19272-1-AP 1:500) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 / TNFRSF1B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 / TNFRSF1B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19272-1-AP

  Immunofluorescent analysis of Hela cells, using TNFR2 / TNFRSF1B antibody 19272-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 19272-1-AP

  1X10^6 HeLa cells were stained with 0.5ug TNFR2 / TNFRSF1B antibody (19272-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells, HEK-293-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, Ratten-Thymusgewebe, THP-1-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• FC: See 1 publications below

Produktinformation

19272-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TNFR2 / TNFRSF1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFR2 / TNFRSF1B fusion protein Ag5866
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC052977
• Gene symbol: TNFRSF1B
• Gene ID (NCBI): 7133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Publikationen

Species	Application	Title
human	FC,IF	Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Mol Ther; Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities.; Authors - Yuechao Zhao
rat	WB	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
rat	WB	Oxid Med Cell Longev; Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats.; Authors - Marwan Almoiliqy
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
mouse	IF	Pain; Nox2-dependent signaling between macrophages and sensory neurons contributes to neuropathic pain hypersensitivity.; Authors - Wiebke Kallenborn-Gerhardt

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tinne (Verified Customer) (08-14-2023)

This worked really well for ICC of human HPCs.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Human hippocampal progenitor cells

TNFR2 / TNFRSF1B Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:19272-1-AP)

Verwandte Produkte

TNFR1 Polyclonal antibody

21574-1-AP

TNFR1 Monoclonal antibody

60192-1-Ig

TNF Alpha Monoclonal antibody

60291-1-Ig

cIAP1 Polyclonal antibody

10022-1-AP

cIAP2 Polyclonal antibody

24304-1-AP