TNFR2/CD120b Polyklonaler Antikörper

TNFR2/CD120b Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 28746-1-AP

Synonyme

TNFR2 / TNFRSF1B, TNFRSF1B, CD120b, Etanercept, p75

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  WB analysis using 28746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 28746-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 28746-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TNFR2 / TNFRSF1B antibody (28746-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

28746-1-AP bindet in WB, IHC, IF/ICC, ELISA TNFR2/CD120b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFR2/CD120b fusion protein Ag30490
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 65 kDa
• GenBank-Zugangsnummer: BC052977
• Gene symbol: TNFR2
• Gene ID (NCBI): 7133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Publikationen

Species	Application	Title
human	WB	PLoS Pathog; SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure; Authors - Huilong Li
human	IHC	Oncol Lett; Association between tumor necrosis factor receptor 2 and progression and poor prognosis of tumor stage 2‑3 esophageal squamous cell carcinoma and stratified analysis; Authors - Zifeng Liu
human	IHC,IF	Front Immunol; Integrating machine learning algorithms and multiple immunohistochemistry validation to unveil novel diagnostic markers based on costimulatory molecules for predicting immune microenvironment status in triple-negative breast cancer; Authors - Chao Zhang
human	IF	Am J Pathol; TNF receptor-2 signals clear-cell renal carcinoma proliferation via phosphorylated-4EBP1 and mitochondrial gene translation; Authors - Rafia S Al-Lamki