TNFR2/CD120b Rekombinanter Antikörper

TNFR2/CD120b Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230328B6

Kat-Nr. : 83101-1-RR

Synonyme

TNFRSF1B, 230328B6, CD120b, Etanercept, p75

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  WB analysis using 83101-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83101-1-RR (TNFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83101-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83101-1-RR against Human TNFR2 were performed. The affinity constant is 0.43 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, K-562-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83101-1-RR bindet in WB, ELISA TNFR2/CD120b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNFR2/CD120b fusion protein Eg0086
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 65 kDa
• GenBank-Zugangsnummer: BC052977
• Gene symbol: TNFR2
• Gene ID (NCBI): 7133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).