TNFR2/CD120b Polyklonaler Antikörper

TNFR2/CD120b Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28746-1-PBS

Synonyme

TNFR2 / TNFRSF1B, TNFRSF1B, CD120b, Etanercept, p75

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  WB analysis using 28746-1-AP (same clone as 28746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 28746-1-AP (same clone as 28746-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 28746-1-AP (same clone as 28746-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 28746-1-AP (same clone as 28746-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TNFR2 / TNFRSF1B antibody (28746-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28746-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TNFR2/CD120b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFR2/CD120b fusion protein Ag30490
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 1B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 65 kDa
• GenBank-Zugangsnummer: BC052977
• Gene symbol: TNFR2
• Gene ID (NCBI): 7133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).