Osteoprotegerin/TNFRSF11B Polyklonaler Antikörper

Osteoprotegerin/TNFRSF11B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 11534-1-AP

Synonyme

Tumor necrosis factor receptor superfamily member 11B, TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG

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  WB analysis of human milk using 11534-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human milk using 11534-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 11534-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 11534-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11534-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Osteoprotegerin antibody (11534-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human milk
• Erfolgreiche Detektion in IHC: Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

11534-1-AP bindet in WB, IHC, IF/ICC, ELISA Osteoprotegerin/TNFRSF11B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Osteoprotegerin/TNFRSF11B fusion protein Ag2139
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 11b
• Berechnetes Molekulargewicht: 401 aa, 46 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC030155
• Gene symbol: TNFRSF11B
• Gene ID (NCBI): 4982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promoting bone formationis. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

Publikationen

Species	Application	Title
human	WB	Anal Cell Pathol (Amst); Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy; Authors - Pei-Long Liu
rat,human	IHC	BMC Oral Health; Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats; Authors - Jinghong Luo
	WB	Foods; Optimization of the Preparation Process and Ameliorative Efficacy in Osteoporotic Rats of Peptide-Calcium Chelates from Skipjack Tuna (Katsuwonus pelamis) Meat; Authors - Wan-Zhen Yan
rat	WB	J Ethnopharmacol; Regulation of monocyte polarization through Nuclear Factor Kappa B /Inhibitor of Kappa B Alpha pathway by Cuscuta chinensis Lam. in postmenopausal osteoporosis; Authors - Xiaochen Li