TNFRSF13B/CD267 Rekombinanter Antikörper

TNFRSF13B/CD267 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250265D1

Kat-Nr. : 85902-1-RR

Synonyme

CD267, Taci, Tnfrsf13b, Transmembrane activator and CAML interactor, Tumor necrosis factor receptor superfamily member 13B

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  WB analysis using 85902-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85902-1-RR (TNFRSF13B/CD267 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A20 using 85902-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using TNFRSF13B/CD267 antibody (85902-1-RR, Clone: 250265D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A20 cells
• Erfolgreiche Detektion in IF/ICC: A20 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85902-1-RR bindet in WB, IF/ICC, ELISA TNFRSF13B/CD267 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNFRSF13B/CD267 fusion protein Eg1814
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 13b
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: NM_021349.1
• Gene symbol: Tnfrsf13b
• Gene ID (NCBI): 57916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B), also known as CD267 or TACI, is a transmembrane protein of the TNF receptor superfamily found predominantly on B cells (PMID: 10920230). TACI is also expressed on activated T cells, myeloma cells, and expressed by monocytes intracellularly at a low level (PMID: 11429548; 16825497). TNFRSF13B is a receptor for TNFSF13/APRIL/CD256 and TNFSF13B/BAFF/CD257 and mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-κB and AP-1 (PMID: 10956646; 10973284; 9311921). It is involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.