CD30/TNFRSF8 Rekombinanter Antikörper

CD30/TNFRSF8 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241330G11

Kat-Nr. : 84241-2-PBS

Synonyme

CD30, 241330G11, CD30L receptor, D1S166E, Ki 1

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  WB analysis of Karpas-299 using 84241-2-RR (same clone as 84241-2-PBS)

  Karpas-299 cells were subjected to SDS PAGE followed by western blot with 84241-2-RR (CD30/TNFRSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84241-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01137-1

  Sandwich ELISA standard curve of MP01137-1, Human CD30/TNFRSF8 Recombinant Matched Antibody Pair - PBS only. 84241-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02334. 84241-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL.

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  Sample test of MP01137-1

  Serum of six individual healthy human donors was measured. The CD30/TNFRSF8 concentration of detected samples was determined to be 1,639.1 pg/mL with a range of 1,021.2-2,597.7 pg/mL.

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  Cytometric bead array standard curve of MP01137-1

  Cytometric bead array standard curve of MP01137-1, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-4-PBS. Standard: RP02334. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84241-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84241-2-RR against Human CD30/TNFRSF8 were performed. The affinity constant is 0.391 nM.

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Geprüfte Anwendungen

Produktinformation

84241-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD30/TNFRSF8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 8
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_001243
• Gene symbol: CD30
• Gene ID (NCBI): 943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).