TNFSF11 Rekombinanter Antikörper

TNFSF11 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241791B7

Kat-Nr. : 84431-5-PBS

Synonyme

241791B7, CD254, ODF, OPGL, Osteoclast differentiation factor

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  WB analysis using 84431-5-RR (same clone as 84431-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84431-5-RR (TNFSF11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84431-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84431-5-RR (same clone as 84431-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84431-5-RR (TNFSF11 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84431-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84431-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84431-5-RR against Mouse TNFSF11 were performed. The affinity constant is 9.78 nM.

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Geprüfte Anwendungen

Produktinformation

84431-5-PBS bindet in WB, IHC, Indirect ELISA TNFSF11 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNFSF11 fusion protein Eg1956
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 11
• Berechnetes Molekulargewicht: 35KD
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_011613.3
• Gene symbol: Tnfsf11
• Gene ID (NCBI): 21943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF11, known as Tumor Necrosis Factor Superfamily Member 11, also known as RANKL (Receptor Activator of Nuclear Factor-κB Ligand), TRANCE, etc., is a member of the Tumor Necrosis Factor (TNF) superfamily.RANKL is a type II homotrimeric transmembrane protein located primarily at the plasma membrane. RANKL is a type II homotrimeric transmembrane protein located mainly at the plasma membrane, and in addition to the membrane-bound form (mRNAKL), it can also be cleaved by proteolytic cleavage of the cell membrane to produce the soluble form (sRANKL). The membrane-bound form of RANKL is the main form involved in triggering the RANKL/RANK signaling pathway and mediating osteoclastogenesis. Current studies have shown that TNFSF11 acts primarily in the immune and skeletal systems and has been implicated in the pathogenesis of degenerative bone diseases, carcinogenesis, and asthma.