TNFSF11 Rekombinanter Antikörper

TNFSF11 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

241791B7

Kat-Nr. : 84431-5-RR

Synonyme

241791B7, CD254, ODF, OPGL, Osteoclast differentiation factor

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  WB analysis using 84431-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84431-5-RR (TNFSF11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84431-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84431-5-RR (TNFSF11 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84431-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84431-5-RR against Mouse TNFSF11 were performed. The affinity constant is 9.78 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84431-5-RR bindet in WB, IHC, ELISA TNFSF11 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNFSF11 fusion protein Eg1956
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 11
• Berechnetes Molekulargewicht: 35KD
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_011613.3
• Gene symbol: Tnfsf11
• Gene ID (NCBI): 21943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF11, known as Tumor Necrosis Factor Superfamily Member 11, also known as RANKL (Receptor Activator of Nuclear Factor-κB Ligand), TRANCE, etc., is a member of the Tumor Necrosis Factor (TNF) superfamily.RANKL is a type II homotrimeric transmembrane protein located primarily at the plasma membrane. RANKL is a type II homotrimeric transmembrane protein located mainly at the plasma membrane, and in addition to the membrane-bound form (mRNAKL), it can also be cleaved by proteolytic cleavage of the cell membrane to produce the soluble form (sRANKL). The membrane-bound form of RANKL is the main form involved in triggering the RANKL/RANK signaling pathway and mediating osteoclastogenesis. Current studies have shown that TNFSF11 acts primarily in the immune and skeletal systems and has been implicated in the pathogenesis of degenerative bone diseases, carcinogenesis, and asthma.

Publikationen

Species	Application	Title
		Exp Cell Res; Activated NETosis of bone marrow neutrophils up-regulates macrophage osteoclastogenesis via cGAS-STING/AKT2 pathway to promote osteoporosis; Authors - Yutong Guo