BAFF/TNFSF13B Polyklonaler Antikörper

BAFF/TNFSF13B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11862-1-AP

Synonyme

TNFSF13B, CD257, BLYS, B-cell-activating factor, BAFF

---

• 

  WB analysis of U-937 using 11862-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11862-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11862-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11862-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11862-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 11862-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11862-1-AP (TNFSF13B/BAFF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, humanes Lebergewebe, Mauslebergewebe, Maushodengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11862-1-AP bindet in WB, IHC, ELISA BAFF/TNFSF13B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAFF/TNFSF13B fusion protein Ag2440
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 13b
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 45-52 kDa
• GenBank-Zugangsnummer: BC020674
• Gene symbol: TNFSF13B
• Gene ID (NCBI): 10673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF13B, also known as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. This cytokine is expressed in B lineage cells, and acts as a potent B cell activator. The BAFF system promotes B cell survival and differentiation and plays a prominent role in the pathogenesis of autoimmune diseases. It has been shown that BAFF inhibitors are in clinical trials for systemic lupus erythematosus with significant efficacy. This antibody recognizes all the forms of TNFSF13B such as soluble form (17 kDa), membrane form (32-35 kDa) and glycosylation form (45-52 kDa).