TNFSF15 Rekombinanter Antikörper

TNFSF15 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251018A11

Kat-Nr. : 83700-3-RR

Synonyme

TNFSF 15, Tumor necrosis factor ligand superfamily member 15, Tumor necrosis factor ligand superfamily member 15, membrane form, Tumor necrosis factor ligand superfamily member 15, secreted form

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  WB analysis using 83700-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83700-3-RR (TNFSF15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83700-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83700-3-RR against Human TNFSF15 were performed. The affinity constant is 0.291 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83700-3-RR bindet in WB, ELISA TNFSF15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNFSF15 fusion protein Eg32092
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 15
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC074941
• Gene symbol: TNFSF15
• Gene ID (NCBI): 9966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor necrosis factor ligand superfamily member 15 (TNFSF15) is also named as TL1 and VEGI. And it belongs to the tumor necrosis factor family. It is a receptor for TNFRSF25 and TNFRSF6B. VEGI induced degradation of IκB alpha, and nuclear translocation of p65 subunit of NFκB by activating NFκB (PMID: 10597252). TL1A mediates activation and apoptosis of NFκB in DR3-expressing cell lines (PMID: 11911831). Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression. The isoforms show endothelial cell-specific expression and are generated from a 17 kb human gene by alternative splicing (PMID: 11923219). In addition, VEGI can inhibit vascular endothelial growth and angiogenesis (in vitro) (PMID: 9872942).