TNFSF18 Polyklonaler Antikörper

TNFSF18 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23899-1-AP

Synonyme

AITRL, GITRL, hGITRL, TL6, TNFSF18

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  WB analysis using 23899-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 23899-1-AP (TNFSF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 23899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 23899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 23899-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 23899-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 23899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 23899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23899-1-AP (TNFSF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:500
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

23899-1-AP bindet in WB, IHC, IF, ELISA TNFSF18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFSF18 fusion protein Ag20979
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 18
• Berechnetes Molekulargewicht: 177 aa, 20 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC069319
• Gene symbol: TNFSF18
• Gene ID (NCBI): 8995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IF,FC	Cell Death Dis; GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt.; Authors - Yu He
mouse	IF	Cell Death Dis; GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice; Authors - Li Li
human	IHC	Am J Surg Pathol; High FCGR2B Expression Can Identify Low-tumor-burden Follicular Lymphoma Patients Who Do Not Require Any Antilymphoma Therapy for a Long Term; Authors - Shotaro Watanabe