TNIK Polyklonaler Antikörper

TNIK Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30906-1-AP

Synonyme

EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

---

• 

  WB analysis using 30906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of K-562 using 30906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (30906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 205 cells, HT-29-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30906-1-AP bindet in WB, IF/ICC, ELISA TNIK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNIK fusion protein Ag34132
• Vollständiger Name: TRAF2 and NCK interacting kinase
• Beobachtetes Molekulargewicht: 155~180 kDa
• GenBank-Zugangsnummer: BC150256
• Gene symbol: TNIK
• Gene ID (NCBI): 23043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNIK (Traf2- and Nck-interacting kinase) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension, which had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling (PMID: 24566388). TNIK is also an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462). Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).