TNKS Polyklonaler Antikörper

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• KD/KO Validated

TNKS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18030-1-AP

Synonyme

PARP 5a, PARP5A, PARPL, TANK1, Tankyrase 1, Tankyrase I, TIN1, TINF1, TNKS, TNKS 1, TNKS1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18030-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18030-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18030-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 18030-1-AP

  IP Result of anti-TNKS (IP:18030-1-AP, 4ug; Detection:18030-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of mouse testis using 18030-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18030-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18030-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18030-1-AP

  Immunofluorescent analysis of HepG2 cells, using TNKS antibody 18030-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18030-1-AP bindet in WB, IP, IHC, IF, ELISA TNKS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNKS fusion protein Ag12597
• Vollständiger Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
• Berechnetes Molekulargewicht: 1327 aa, 142 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC098394
• Gene symbol: TNKS
• Gene ID (NCBI): 8658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC, WB, IF	Oncol Lett; Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.; Authors - Yanmei Ma
human	WB,IHC	Cell Death Dis; circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.; Authors - Zhifei Ma ; KD Validated
human	WB	Adv Sci (Weinh); Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis; Authors - Hyeoncheol Kim
human	WB	Chem Biol Interact; Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer; Authors - Rong Gong
		Biochim Biophys Acta Mol Basis Dis; Iron promotes glycolysis to drive colon tumorigenesis; Authors - Zhaoli Liu